type hct116  (ATCC)

Journal: Biosensors

Article Title: Hybrid Oxygen-Sensing Bio-Scaffolds for 3D Micro-Tissue Models

doi: 10.3390/bios16020122

Figure Lengend Snippet: Dose- and time-dependent profiles of toxicity of the Matrigel–Nano-IR hybrid scaffolds on dispersed HCT116 cells (6k cells in 6 µL aliquots). ( A , B ) show dependence of cell viability on probe concentrations and incubation time, respectively. Liquid scaffold with cells was incubated for 1 h at 37 °C to solidify, then 100 µL of DMEM medium was applied on top. After further incubation (specified), the cells were analysed on a plate reader for total ATP levels using the CellTiter-Glo kit. Results were normalised to the levels of ATP in cells dispersed in Matrigel scaffold without Nano-IR probe.

Article Snippet: Wild-type HCT116 cells were obtained from the American Tissue Culture Collection, ATCC (Manassas, Virginia, VA, USA).

Techniques: Incubation

Journal: Biosensors

Article Title: Hybrid Oxygen-Sensing Bio-Scaffolds for 3D Micro-Tissue Models

doi: 10.3390/bios16020122

Figure Lengend Snippet: Total ATP levels (chemiluminescence counts) for spheroids of HCT116 cells cultured in the hybrid scaffolds containing 0.625 mg/mL Nano-IR probe. CV = 6.83% and 2.84% for spheroids produced by seeding 2k and 4k cells per Lipidure ® coated well. ATP was measured as in .

Article Snippet: Wild-type HCT116 cells were obtained from the American Tissue Culture Collection, ATCC (Manassas, Virginia, VA, USA).

Techniques: Cell Culture, Produced

Journal: Biosensors

Article Title: Hybrid Oxygen-Sensing Bio-Scaffolds for 3D Micro-Tissue Models

doi: 10.3390/bios16020122

Figure Lengend Snippet: Phosphorescent signals for Matrigel–Nano-IR (1 mg/mL) hybrid scaffolds with cultured HCT116 spheroids (2k and 4k, three samples A , C , D in each batch), measured on a wide-field PLIM microscope . ( A ): Two-dimensional intensity images; ( B ): representative line profile of the intensity signal across the spheroid; ( C ): local lifetime values in the different regions of the sample. Positions of the samples on the plate are shown (A-1, C-1, …). Pair t -test analysis: p < 0.01 for the lifetime signal in areas distant and adjacent to the spheroid cultured in the Matrigel–Nano-IR scaffold.

Article Snippet: Wild-type HCT116 cells were obtained from the American Tissue Culture Collection, ATCC (Manassas, Virginia, VA, USA).

Techniques: Cell Culture, Microscopy

Journal: Biosensors

Article Title: Hybrid Oxygen-Sensing Bio-Scaffolds for 3D Micro-Tissue Models

doi: 10.3390/bios16020122

Figure Lengend Snippet: Phosphorescence lifetime profiles of the HCT116 cell spheroids seeded in 6 mL of 50% Matrigel scaffold containing 1 mg/mL of NanO2 probe and covered with 100 mL of McCoy medium, measured on a Victor2 plate reader in TR-F mode. After temperature equilibration of the plate at 37 °C, 100 μL of oil was applied on top of the sample (at ~20 min) to limit back diffusion of O 2 from air.

Article Snippet: Wild-type HCT116 cells were obtained from the American Tissue Culture Collection, ATCC (Manassas, Virginia, VA, USA).

Techniques: Diffusion-based Assay